A series of nearisogenic glycinebetainecontaining and deficient f8 pairs of zea mays l. Crop science abstract nearisogenic barley lines with genes. What is difference between isogenic and near isogenic line. The pairs of lines differ for alternative alleles of a single locus. Nearisogenic lines for measuring phenotypic effects of. Bx10c inactivation causes high dimboaglc, low hdmboaglc, and decreased aphid reproduction relative to nearisogenic lines that have a functional bx10c gene. Here, through multiple rounds of backcrossing and spinosad screening, we generated spinosad resistant nils in the western flower thrips, frankliniella occidentalis pergande thysanoptera. Zinc effects on cadmium accumulation and partitioning in near. Bx10c inactivation causes high dimboaglc, low hdmboaglc, and decreased aphid reproduction relative to near isogenic lines that have a functional bx10c gene.
In classical plant breeding this means that vegetatively propagated clones are isogenic and lines of inbreeders become isogenic after repeated selffertilization. The important difference was in kernel weight with purple isogenic lines being significantly higher than the redgrained line. The dkc stands for dekalb seeds, which is owned by monsanto, and the numbers are the identifiers used by the company to denote the line. Alteration of antioxidant enzyme gene expression during cold. The near isogenic lines nils inbred lines are usually developed for qtl mapping purpose. Understanding patterns in bph virulence against resistance genes is necessary to. For example, in the maize genetics community the set of 302 ibm maize strains has been a cornerstone of both basic and applied research sharopova et al. Isogenic article about isogenic by the free dictionary. Isogenic human disease models are a family of cells that are selected or engineered to accurately model the genetics of a specific patient population, in vitro. A series of near isogenic glycinebetainecontaining and deficient f8 pairs of zea mays l. The nearisogenic lines nils inbred lines are usually developed for qtl mapping purpose. To this effect, two gm maize varieties gm bt, gm rr, each containing a single insert, were subjected to comparative profiling, using the near.
With the aim of determining the type of symbiotic relationship between bpev and bell pepper, we evaluated some morphological and physiological characters of these two nils. They can be used to model any disease with a genetic foundation. Deciphering signalling network in broad spectrum near. The structural periodicity of semicrystalline starch granules is formed by alternating crystalline and amor phous layers lamellae. Two bell pepper nearisogenic lines nils, one bpevinfected and the other bpev free, were generated and used to investigate the type of symbiotic relationship between bpev and bell pepper. We constructed a population of 92 introgression lines carrying between one and four cvi introgression fragments in a ler genetic background. Nearisogenic lines are the most convenient objects for a wide range of biological and agricultural experiments. The lipoxygenase free lines were verified using seed chips in a colormetric laboratory assay modified after suda et al. Quantitative trait locus mapping using sets of nearisogenic lines. Nearisogenic lines of maize differing for glycinebetaine. Pdf registration of four nearisogenic soybean lines of. Pdf registration of four nearisogenic soybean lines of g00. Nearisogenic lines can be derived through repeated backcrossing to a recurrent parent.
Breeding programs for purple wheat are underway in many countries but there is a lack of information on the effects of pp purple pericarp genes on agronomic and quality traits in variable environments and along the product chain grainflourbread. Alteration of antioxidant enzyme gene expression during. Recombinant inbred lines or near isogenic lines have been developed in many different species during the last 50 years. Alteration of antioxidant enzyme gene expression during cold acclimation of near isogenic wheat lines kwanghyun baeka, daniel z. To survey bph virulence patterns, seven nearisogenic lines nils, each with a single bph resistance gene bph2nil, bph3nil, bph17nil, bph20nil. Recombinant inbred lines or nearisogenic lines have been developed in many different species during the last 50 years. Construction and evaluation of nearisogenic lines for major. This work describes the development of maize recombinant near isogenic lines nils for the mendelization of six heterotic qtl previously identified based on a maize zea mays l. However, the effectiveness of resistance genes varies spatially and temporally according to bph virulence. Construction of a genomewide near isogenic line population. The lines offered one year may not be the same ones offered the next. In that case, b concentrations in shoots sampled at booting and grain maturity were significantly lower in the near isogenic line btschomburgk than the parent moody et al.
Development and evaluation of nearisogenic lines for brown. Jun 17, 2011 numerous quantitative trait loci qtl have been previously mapped using a recombinant inbred line population derived from these two genotypes. Yield and quality in purplegrained wheat isogenic lines. In this investigation, we applied previously proposed simple algorithms to analyze the attenuated total reflection fourier transform infrared atr ftir spectra of cotton fibers during secondary cell wall scw biosynthesis. Construction of a genomewide nearisogenic line population. Development and evaluation of nearisogenic lines for brown planthopper resistance in rice cv. Isogenic cell lines clinically relevant cell models are critical for studies of molecular and cellular mechanisms of tumors, as well as for drug screening for cancer. Pdf nearisogenic lines nils constitute valuable tools in genetic. With genome editing tools such as crisprcas9, atcc has created isogenic cell lines with mutants of key oncogenes, which are ideal for identifying novel, personalized treatment. Nearisogenic lines in a parthenogenetically reproduced thrips species, frankliniella occidentalis. Host plant resistance has tremendous potential to reduce the damage caused to rice by the planthopper.
Near isogenic lines confirm a soybean cyst nematode. This is a new line that differs from its parent in only one genomic location. Achieving this is nearly impossible, so researchers will use nearisogenic lines genetically these are at least 99% identical. Near isogenic lines are frequently the product of plant breeding programs. Numerous quantitative trait loci qtl have been previously mapped using a recombinant inbred line population derived from these two genotypes. The efficient and inexpensive strategy adopted to generate sets of nils. Although nearisogenic lines nils can standardize genetic. Nearisogenic lines of triticum aestivum with distinct modes of resistance exhibit dissimilar transcriptional regulation during diuraphis noxia feeding, biology open, 2014, pp. Cereals are among the most important group of cultivated plants for food production worldwide. A multiple near isogenic line multinil rnaseq approach to identify candidate genes underpinning qtl november 28, 2017 leave a comment 2,100 views this study demonstrates how identification of genes underpinning diseaseresistance qtl based on differential expression and snps can be improved by performing transcriptomic analysis on multiple. Skinnera,b, a department of crop and soil sciences, 210 johnson hall, washington state university, pullman, wa 991646420, usa b usdaars, department of crop and soil sciences, 209 johnson hall, washington state. Total belowground co 2 was trapped and partitioned for determination of soil organic c decomposition and rpe using a stable isotopic tracing technique.
These two parents were used to produce a set of near isogenic lines nils with small regions of introgression into both backgrounds. The overall appearance of the two nils was similar. Transcriptome analysis of near isogenic lines provides molecular insights into starch biosynthesis in maize kernel yingni xiao1, shawn thatcher2, min wang1,3, tingting wang1, mary beatty4, gina zastrowhayes4, lin li5, jiansheng li1, bailin li2 and xiaohong yang1 1national maize improvement center of china, beijing key laboratory of crop genetic improvement, china agricultural university, beijing. Nearisogenic lines of triticum aestivum with distinct. View the article pdf and any associated supplements and figures for a period of 48 hours.
The development and characterization of nearisogenic and. Generally speaking, nearisogenic lines nil are not available for purchase and must be generated. Development of nearisogenic lines in a parthenogenetically. Near isogenic lines have been used to understand the relationships between the wheat protein composition and functional quality. The power to detect qtl using nearisogenic line nil comparisons versus recombinant inbred ri populations was assessed. Development of near isogenic lines nils using these type of broad. Construction and evaluation of nearisogenic lines for major qtls of basal root thickness and grainweight in lowland and upland rice volume 4 issue 3 liu li. Multiple nearisogenic lines targeting a qtl hotspot of.
These two parents were used to produce a set of nearisogenic lines nils with small regions of introgression into both. Please view the pdf by using the full text pdf link under view to the left. These numbers are akin to a catalog number used to sell seed. Near isogenic lines confirm a soybean cyst nematode resistance gene from pi 88788 on linkage group j k.
Near isogenic lines, with near identical genetic background but varying mainly in the alleles responsible for a trait of interest, are useful in identification of target genes and pathways. To characterize the phenotypic effects of dimboaglc methyltransferase activity, we created nearisogenic lines derived from b73 and cml277 that do or do not contain the transposon insertion. Transcriptome analysis of nearisogenic lines provides. Here, transcriptome analysis of immature spike tissues in three lines nearly isogenic for q revealed. Such lines can be created by crossing a donor line containing a gene or trait of interest with a recurrent parent to produce a heterozygous f1, and then repeatedly backcrossing the offspring to the recurrent parent bc1, bc2, etc, retaining the donor gene or trait in each successive generation. Pairs of nearisogenic lines nils with a common genetic.
Nearisogenic lines nils, pairs of lines that are identical except for a single genomic segment, are ideal for this purpose szalma et al. In this study, nearisogenic lines nils for 17 drought resistancerelated qtl were constructed and phenotypic variations of these nils were investigated under drought and normal conditions. Recombinant inbred lines developed from the maize zea mays ssp. Pdf highresolution mapping of a gene conferring strong. Isogeneic definition of isogeneic by medical dictionary. Rhizosphere priming of two nearisogenic wheat lines varying. Improving groundnut using near isogenic lines for the first time 20 may 2016 for the first time in groundnut crop, a near isogenic lines nils evaluation trial of marker assisted backcrossing mabc derived lines, was constituted. Frontiers comparative transcriptomic analysis of two. The list of acronyms and abbreviations related to nil near isogenic line.
A set of lines that are genetically identical, except for one or a few loci. Isogenic definition of isogenic by medical dictionary. Isogenic lines are defined as individuals that possess the same genotype irrespective of their homo or heterozygous nature johannsen, 1926. These reselections could be considered nearisogenic lines nils for genes ltn and lr34 because cultivar huw234 originated from a single back cross huw12. Genetically, these lines differ by only a few genes but are identical beyond that. Log in to wiley online library new phytologist trust. Two nearisogenic lines nils were generated, one bpevinfected and the other bpev free. Near isogenic lines are generated by a process of repeated backcrossing. In this study, near isogenic lines nils for 17 drought resistancerelated qtl were constructed and phenotypic variations of these nils were investigated under drought and normal conditions.
The most popular abbreviation for near isogenic line is. Development of a nearisogenic line population of arabidopsis. They are provided with a genetically matched normal cell to provide an isogenic system to research disease biology and novel therapeutic agents. Use of nearisogenic wheat lines to determine the glutenin. The presence of the insect resistant qtls in the nearisogenic lines benning m, benning g, benning h, and benning mgh were verified using ssr simple sequence repeat molecular markers. Nov 15, 2019 disease resistance r genes like pi9, pita, pi21, pi54 are playing important role for broad spectrum blast resistance in rice. Evaluation of pest vulnerability of benning soybean. Development and evaluation of nearisogenic lines for. Improving groundnut using nearisogenic lines for the first time 20 may 2016 for the first time in groundnut crop, a nearisogenic lines nils evaluation trial of marker assisted backcrossing mabc derived lines, was constituted. Selected morphological and physiological characteristics of the nils were evaluated.
Near isogenic lines are generated by a process of repeated. These varieties were compared in a single location, petit, during three consecutive years. Nearisogenic lines nils a good way of validating a putative qtl is by creating a nearisogenic line nil. This was an increase of 4 to 5% in favor of the purplegrained lines. Construction and evaluation of nearisogenic lines for. B73mo17 nearisogenic lines demonstrate dispersed structural variation in maize1woa steven r. Here we examined several physiological properties of two near. As such, they are also useful for understanding the functional contribution of that. Disease resistance r genes like pi9, pita, pi21, pi54 are playing important role for broad spectrum blast resistance in rice. To survey bph virulence patterns, seven near isogenic lines nils, each with a single bph resistance gene bph2nil, bph3nil, bph17nil, bph20nil, bph21nil, bph32nil and bph17ptbnil and fifteen pyramided lines pyls carrying multiple resistance genes were developed with the genetic background of the japonica rice variety, taichung 65. Genomic and phenotypic characterization of a wild medaka.
Using the marker identified for that qtl, backcrosses are made to the recurrent parent usually the cultivated variety, in a wide cross. Plants were grown in solution culture as described previously hart et al. Apr 26, 2017 a set of lines that are genetically identical, except for one or a few loci. Rhizosphere priming of two nearisogenic wheat lines. Genetic engineering of agricultural crops has played an important role in crop improvement where it has been used to increase resistance to. Koornneef, 2003 analysis of natural allelic variation at seed dormancy loci of arabidopsis thaliana. For example, in the maize genetics community the set of 302 ibm maize strains has been a cornerstone of both basic and applied research. Joint analysis of nearisogenic and recombinant inbred. Near isogenic lines nils near isogenic lines are frequently the product of plant breeding programs where the objective has been the introgression of a desirable character or trait gene from a donor parent into an otherwise agronomically acceptable cultivar recurrent parent. However, little information is known regarding the molecular mechanisms associated with the regulation of these traits by q, especially for the structural. Although nearisogenic lines nils can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. To develop the nil mapping population, cross of a donor line with a trait of interest and a reference. Isogenic definition is characterized by essentially identical genes. What are isogenic lines and why should they be used to.
Near isogenic lines are developed to transfer a single gene or loci through backcrossing into a common genetic background. Many achievements in the fields of plant immunity and pest resistance became possible only due to substitution of conventional testers with nils. The nearisogenic lines were used to investigate whether glycinebetaine. Ir64 with accumulation of provitamin a in the endosperm tissue. Most r genes to blb have been introgressed into the background of the susceptible indica cultivar to develop a set of near isogenic lines nils, and some have been pyramided, either through classical breeding and markerassisted selection or through genetic engineering, to develop new plant types and nils sanchez et al. Highresolution mapping of a gene conferring strong antibiosis to brown planthopper and developing resistant nearisogenic lines in 9311 background. Although near isogenic lines nils can standardize genetic backgrounds among individuals, it has never been applied in parthenogenetically reproduced animals. To characterize the phenotypic effects of dimboaglc methyltransferase activity, we created near isogenic lines derived from b73 and cml277 that do or do not contain the transposon insertion. These reselections could be considered near isogenic lines. Near isogenic lines nils a good way of validating a putative qtl is by creating a near isogenic line nil. These nearisogenic barley lines are recommended for both practical and theoretical research work in the barleypowdery mildew system. Transcriptomic profiling of melilotus albus nearisogenic. Although heterosis is widely exploited in agriculture, a clear understanding of its genetic bases is still elusive. Construction and evaluation of near isogenic lines for major qtls of basal root thickness and grainweight in lowland and upland rice volume 4 issue 3 liu lifeng, zhang hongliang, mu ping, qu yanying, li zichao.
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